LFIRE™
In Label-Free Internal Reflection Ellipsometry™, the principles of ellipsometry and evanescent wave detection are applied to quantify the degree of specific binding of biomolecules in a sample to the capture chemistry on a microarray or plate. In the LFIRE™ platform, the underside of a microarray chip or multi-well plate is illuminated with elliptically polarized light. The beam reflects from the solid-liquid interface without propagating into the liquid. When molecules enter the evanescent field, the polarization of the reflected beam shifts in a precise, predictable way. As analyte "probes" in the sample are specifically captured by the "target" molecules on the surface, the density of molecules in the evanescent field region increases, creating a dynamic polarization shift proportional to the surface density of captured probes. The noise in this signal is equivalent to 1 pg/mm2 of protein on the surface.
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